{"product_id":"nad","title":"NAD+","description":"\u003cdiv class=\"ds-virtual-list-items _6f2c522\"\u003e\n\u003cdiv class=\"ds-virtual-list-visible-items\"\u003e\n\u003cdiv class=\"_4f9bf79 d7dc56a8 _43c05b5\"\u003e\n\u003cdiv class=\"ds-message _63c77b1\"\u003e\n\u003cdiv class=\"ds-markdown ds-assistant-message-main-content\"\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cspan class=\"\"\u003eCAS 53-84-9. \u0026gt;99% purity by RP‑HPLC at 260 nm. 1000 mg of lyophilized, oxidized β‑Nicotinamide Adenine Dinucleotide packed under argon in an amber glass vial. \u003ca href=\"https:\/\/corepeptides.in\/\"\u003eCorePeptides India\u003c\/a\u003e delivers every gram with a batch‑locked Certificate of Analysis.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan class=\"\"\u003eProduct Specifications\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv class=\"ds-scroll-area ds-scroll-area--show-on-focus-within ds-scroll-area--enabled _1210dd7 c03cafe9\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cspan class=\"\"\u003eSpecification\u003c\/span\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cspan class=\"\"\u003eDetail\u003c\/span\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003eProduct Class \/ Category\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003ePyridine Nucleotide Coenzyme (Oxidised Form)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003eResearch Identifier\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003eβ‑Nicotinamide Adenine Dinucleotide (NAD⁺, NAD, Diphosphopyridine Nucleotide), CAS 53‑84‑9\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003eSupplier \/ Brand\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003eCorePeptides India\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003eHPLC Purity Threshold\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003e\u0026gt;99% (RP‑HPLC, C18, UV‑Vis detection at 260 nm, batch‑specific)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003eTarget Receptors \/ Chemical Pathways\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003eRedox cofactor (hydride acceptor in glycolysis, TCA cycle, oxidative phosphorylation); substrate for sirtuin deacetylases (SIRT1‑7), poly‑ADP‑ribose polymerases (PARPs), CD38\/CD157 NADases; in‑vitro modulator of ADP‑ribosylation, cellular NAD⁺\/NADH ratio, and mitochondrial respiration\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003eRegulatory Status\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan class=\"\"\u003eFor In‑Vitro \u0026amp; Laboratory Analytical Research Only. Not for human, veterinary, diagnostic, or therapeutic use.\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003chr\u003e\n\u003ch2\u003e\u003cspan class=\"\"\u003eResearch Overview \u0026amp; Mechanism of Action\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cspan class=\"\"\u003eNAD⁺ is a dinucleotide built from nicotinamide mononucleotide (NMN) and adenosine monophosphate (AMP), bridged by a pyrophosphate bond. Its oxidised nicotinamide ring absorbs sharply at 260 nm with an extinction coefficient of 17.8 × 10³ M⁻¹cm⁻¹ - a bench‑mark constant every researcher should know. Molecular weight is 663.4 g\/mol. A 1000 mg vial supplies enough material for months of repeatable analytical concentration‑response work without the noise of inter‑lot variability.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cspan class=\"\"\u003eInside a cuvette or a well plate, NAD⁺ accepts a hydride ion and a proton to form NADH. The reduced form gains a distinct absorbance at 340 nm. That spectral shift is the practical readout for countless dehydrogenase‑coupled enzyme assays. Beyond redox, this molecule is an obligatory substrate for NAD⁺‑consuming enzymes - sirtuins strip the nicotinamide moiety to generate O‑acetyl‑ADP‑ribose during deacetylation, while PARPs cleave the glycosidic bond entirely to build poly‑ADP‑ribose chains. Researchers running in‑vitro SIRT1 activity assays or PARP‑1 auto‑modification gels rely on a single key variable: the molar ratio of NAD⁺ to enzyme in the reaction mix. A 1000 mg stock lets you standardise that ratio across experimental blocks without exhausting supply mid‑study. The lyophilized powder often looks a little clumped or electrostatically stuck to the glass. That’s harmless - it’s just the light, fluffy nature of bulk‑dried dinucleotide.\u003c\/span\u003e\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003e\u003cspan class=\"\"\u003eHandling, Reconstitution \u0026amp; Storage Protocol\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cspan class=\"\"\u003eLyophilized NAD⁺ is hygroscopic. Keep the sealed amber vial at –20 °C with desiccant. Short ambient‑temperature transit - under a week - does not trigger detectable degradation in our stability series. Upon arrival, let the unopened vial sit at room temperature for 30 minutes. A one‑gram dry mass holds thermal inertia; opening it cold invites condensation and rapid moisture uptake that can accelerate hydrolysis of the pyrophosphate bond.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cspan class=\"\"\u003eReconstitution for analytical research:\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan class=\"\"\u003eUse ultrapure, degassed water (≥18.2 MΩ·cm) or sterile phosphate‑buffered saline, pH 7.4.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"\"\u003eFor a 100 mM stock, add 15.07 mL of diluent to the 1000 mg powder. Inject the solvent slowly, aiming for the glass wall, then swirl. Don’t vortex. The solution should turn clear within a minute.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"\"\u003eNAD⁺ is acidic in solution; the free acid form will drop unbuffered water to pH ~4. If your enzyme assay demands physiological pH, use PBS or adjust with a small volume of sterile 1M NaOH while monitoring pH - the pyrophosphate linkage is labile above pH 8, so work carefully.\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan class=\"\"\u003eFor sub‑millimolar working concentrations in cell‑culture or enzymatic assays, prepare single‑use aliquots immediately. NAD⁺ in solution undergoes slow non‑enzymatic hydrolysis at room temperature. Freeze aliquots at –80 °C. A single freeze‑thaw cycle can cut enzymatic signal by about 5–10% -tolerable for some screens, but avoid it for rigorous kinetic work.\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003e\u003cspan class=\"\"\u003eScientific References \u0026amp; External Citations\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli class=\"ds-markdown-paragraph\"\u003e\n\u003ca href=\"https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/5893\" rel=\"noreferrer\" target=\"_blank\"\u003e\u003cspan class=\"\"\u003ePubChem Compound: NAD⁺ (CID 5893)\u003c\/span\u003e\u003c\/a\u003e\u003cspan class=\"\"\u003e\u003cspan\u003e -\u003c\/span\u003e Full chemical identifiers, spectral data, and structural files.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"ds-markdown-paragraph\"\u003e\n\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NAD%252B+sirtuin+in+vitro+assay\" rel=\"noreferrer\" target=\"_blank\"\u003e\u003cspan class=\"\"\u003ePubMed: Sirtuin \u0026amp; NAD⁺ Research\u003c\/span\u003e\u003c\/a\u003e\u003cspan class=\"\"\u003e\u003cspan\u003e -\u003c\/span\u003e Peer‑reviewed in‑vitro enzyme studies mapping NAD⁺‑dependent deacetylase activity.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"ds-markdown-paragraph\"\u003e\n\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NAD%252B+redox+assay+mitochondrial\" rel=\"noreferrer\" target=\"_blank\"\u003e\u003cspan class=\"\"\u003ePubMed: NAD⁺ in Cellular Bioenergetics\u003c\/span\u003e\u003c\/a\u003e\u003cspan class=\"\"\u003e\u003cspan\u003e -\u003c\/span\u003e Research protocols using NAD⁺\/NADH ratio measurements in isolated mitochondria and cell lysates.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003e\u003cspan class=\"\"\u003eFrequently Asked Research Questions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003e\u003cspan class=\"\"\u003eWhat is the CAS number and molecular weight of NAD⁺?\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cspan class=\"\"\u003eThe oxidised form, β‑Nicotinamide Adenine Dinucleotide, carries CAS number 53‑84‑9 and has a molecular weight of 663.4 g\/mol. Its characteristic UV absorbance peak at 260 nm (ε = 17.8 × 10³ M⁻¹cm⁻¹) is used to verify concentration and purity in every CorePeptides India batch.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003e\u003cspan class=\"\"\u003eHow should I reconstitute the 1000 mg vial for sirtuin or PARP enzyme assays?\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cspan class=\"\"\u003eDissolve the powder in sterile PBS or ultrapure water to a stock analytical concentration of 100 mM (15.07 mL for the full gram). Let the vial warm completely before opening to avoid moisture condensation. Swirl gently - vortexing is unnecessary. Adjust pH carefully if using water, and aliquot single‑use volumes for –80 °C storage to preserve substrate integrity.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003e\u003cspan class=\"\"\u003eIs a batch‑specific Certificate of Analysis provided by CorePeptides India?\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cspan class=\"\"\u003eYes. Every 1000 mg vial ships with a printed and digitally archived COA showing the RP‑HPLC purity, retention time, UV‑Vis spectrum, and mass confirmation. The lot number on the vial links directly to that COA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003e\u003cspan class=\"\"\u003eCan NAD⁺ be used in human clinical or therapeutic research?\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cspan class=\"\"\u003eNo. CorePeptides India supplies NAD⁺ exclusively for in‑vitro \u003ca href=\"https:\/\/corepeptides.in\/collections\/all\"\u003elaboratory research peptides\u003c\/a\u003e. It is not manufactured for, nor authorised for, human or veterinary administration, diagnostic procedures, or clinical therapeutic development. All purchase orders are screened to uphold this restriction.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Core Supplements","offers":[{"title":"1000mg","offer_id":44198427983931,"sku":null,"price":6500.0,"currency_code":"INR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0696\/6411\/2699\/files\/ChatGPTImageJul20_2026_10_30_36PM.png?v=1789453671","url":"https:\/\/peptidesinindia.org\/products\/nad","provider":"Buy Peptides In India","version":"1.0","type":"link"}