CorePeptides India supplies P21-research identifier P021-as a sterile, lyophilized powder, 5 mg per nitrogen‑flushed vial. Every batch arrives with a fully traceable Certificate of Analysis covering HPLC purity, mass identity, and residual solvent profile.
Product Specifications
| Specification | Detail |
|---|---|
| Product Class / Category | Neurogenic Tetradecapeptide (CNTF-Derived Fragment) |
| Research Identifier / Sequence | P021 (P21); Ac‑Val‑Gly‑Asp‑Gly‑Gly‑Leu‑Phe‑Glu‑Lys‑Lys‑Leu‑NH₂ |
| Supplier / Brand | CorePeptides India |
| HPLC Purity Threshold | >99% (RP‑HPLC, C18, 214 nm, batch‑specific) |
| Target Receptors / Chemical Pathways | CNTF receptor complex (CNTFRα‑gp130‑LIFRβ) — partial in‑vitro binding; downstream upregulation of BDNF expression and inhibition of GSK‑3β‑mediated tau phosphorylation observed in primary neuronal cultures |
| Regulatory Status | For In‑Vitro & Laboratory Analytical Research Only. Not for human, veterinary, diagnostic, or therapeutic use. |
Research Overview & Mechanism of Action
P21 is an acetylated, C‑terminally amidated 11‑mer peptide modelled on residues 147–157 of human ciliary neurotrophic factor. Two substitutions - Asp for Glu at position 3 and Glu for Gln at position 9-were introduced to improve solution stability. The monoisotopic mass is 1176.3 Da. HPLC chromatograms in our laboratory show a single sharp peak; the COA you receive reflects that exact analysis, not a generic lot average.
This peptide does not simply mimic full‑length CNTF. In vitro data point to a biased interaction with the CNTF receptor complex that triggers BDNF transcription without inducing the full JAK/STAT cascade normally seen with CNTF. That makes it a valuable tool for dissecting neurotrophin‑mediated signalling. Researchers use P21 in hippocampal progenitor‑cell differentiation assays, primary cortical‑neuron cultures, and tau‑hyperphosphorylation models. Receptor selectivity is molar‑ratio dependent, so every experiment demands precise analytical concentration control. The lyophilized cake may show minor cracks — a natural consequence of freeze‑drying that leaves molecular integrity untouched.
Handling, Reconstitution & Storage Protocol
Store the unopened vial at –20 °C. Transport at ambient temperature for 3–4 days causes no detectable loss of purity. After shipping, let the vial sit on the bench for 15–20 minutes before opening; this prevents condensation from pulling moisture into the peptide matrix.
Reconstitution for analytical research:
- Use sterile bacteriostatic water (0.9% benzyl alcohol) or sterile PBS, pH 7.4.
- Target a stock analytical concentration of 1 mg/mL. Incline the vial and introduce the diluent slowly down the glass.
- Swirl gently-never vortex. The acetylated N‑terminus and amidated C‑terminus make the peptide reasonably soluble, but mechanical stress shears the backbone.
- For low‑micromolar working solutions in cell‑culture plates, include 0.1% molecular‑biology‑grade BSA as a carrier protein. Polystyrene adsorption can sequester measurable peptide mass and shift the effective molar ratio.
Once reconstituted, keep the solution at 2–8 °C and use within 2‑3 weeks. Single‑use aliquots are strongly recommended. A single freeze‑thaw cycle can reduce detectable signal in ELISA‑based BDNF readouts by roughly 10–15%. Plan your aliquot volumes accordingly.
Scientific References & External Citations
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PubMed: P021 Neurogenic Peptide Research — Peer‑reviewed studies exploring BDNF upregulation, tau phosphorylation inhibition, and neurogenesis in in‑vitro and transgenic Alzheimer’s models.
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ClinicalTrials.gov: P021 Investigator‑Led Studies — Registered clinical research protocols involving the P021 peptide fragment.
Frequently Asked Research Questions
What is the exact sequence and molecular weight of the P21 peptide?
The research peptide supplied by CorePeptides India is P021, sequence Ac‑Val‑Gly‑Asp‑Gly‑Gly‑Leu‑Phe‑Glu‑Lys‑Lys‑Leu‑NH₂. The acetylated, amidated, single‑oxidation form has a monoisotopic mass of 1176.3 Da. This identity is confirmed by ESI‑MS on every batch‑specific Certificate of Analysis.
How should P21 be reconstituted for primary neuron experiments?
Prepare a 1 mg/mL stock in sterile PBS or bacteriostatic water. Add 0.1% BSA when diluting to final working concentrations below 10 µM to minimise adsorptive loss to plasticware. Always allow the lyophilized cake to reach room temperature before adding the diluent to prevent moisture condensation.
Is a batch‑matched Certificate of Analysis provided with every order?
Yes. Every 5 mg vial from CorePeptides India ships with a printed and digitally archived COA showing the HPLC purity, retention time, ESI‑MS spectrum, and observed mass. The lot number on the vial links one‑to‑one with that COA.
Can this peptide be used in human clinical diagnostics or therapeutic development?
No. P21 is manufactured strictly for in‑vitro laboratory research. It is not authorised for human or veterinary administration, diagnostic procedures, or therapeutic use. All orders are verified against this research‑only restriction.
